Imusyn’s Dyemers are novel T cell staining reagents for the detection and enumeration of antigen-specific CD8+ T cells. Dyemers consist of recombinant MHC class I molecules expressed in mammalian cell culture, allowing for authentic glycosylation of the protein. Our recombinant MHC class I is coupled to fluorophore-labeled streptavidin, which is available as PE- or APC-conjugate. By utilizing a streptavidin-binding tag for multimerization, our T cell staining reagent can be easily removed from the cell surface by biotin incubation.
• Expressed in mammalian cell culture
• Off-the-shelf or custom-made Dyemers
• Peptide-receptive myDyemer for self-loading
• Large diversity of alleles available
• Reversible staining
In addition to our ready-to-use Dyemers (see Basic Panel), we offer myDyemers, a peptide-receptive and pre-multimerized staining reagent. Due to our molecular modifications, myDyemers remain stable in a peptide-deficient state and allow you to load your peptide of interest by single-step peptide addition, eliminating the need for time-consuming refolding assays or peptide exchange. Our myDyemer technology facilitates the generation of staining reagents for detection and screening of a large number of different T cell specificities. Paired with the wide range of different MHC class I alleles available at imusyn, we are able to offer T cell staining reagents for virtually any of your need.
Our Dyemers & myDyemers detect antigen-specific CD8+ T cells reliably and stain with a competitive efficiency when compared to other T cell staining reagents, such as tetramers (Figure 1). Convince yourself and order your T cell staining reagent now.
Are you unsure, whether your peptide will bind to certain HLA class I alleles or do you want to verify your binding prediction? Try our Class I Peptide Binding Analysis - imuPep. By loading our recombinant HLA class I molecules with your peptides, we can give information on peptide binding by monitoring the thermal stability of the peptide/HLA class I complex.
Upon request, we can provide you with custom-made Dyemers loaded with your confirmed peptides. For requests, please use our contact form.
All Dyemers & myDyemers in our Basic Panel are available on demand (see below), including peptide/MHC combinations covering the most prominent and widely used EBV and HCMV specificities.
For orders or quotes, please refer to our order form.