Dyemer & myDyemer
Imusyn’s Dyemers are novel T cell staining reagents for the detection and enumeration of antigen-specific CD8+ T cells. Dyemers consist of recombinant MHC class I molecules expressed in mammalian cell culture, allowing for authentic glycosylation of the protein. Our recombinant MHC class I is coupled to fluorophore-labeled streptavidin, which is available as PE- or APC-conjugate. By utilizing a streptavidin-binding tag for multimerization, our T cell staining reagent can be easily removed from the cell surface by biotin incubation.
Features Overview:
• Expressed in mammalian cell culture
• Off-the-shelf or custom-made Dyemers
• Peptide-receptive myDyemer for self-loading
• Large diversity of alleles available
• Reversible staining
In addition to our ready-to-use Dyemers (see Basic Panel), we offer myDyemers, a peptide-receptive and pre-multimerized staining reagent. Due to our molecular modifications, myDyemers remain stable in a peptide-deficient state and allow you to load your peptide of interest by single-step peptide addition, eliminating the need for time-consuming refolding assays or peptide exchange. Our myDyemer technology facilitates the generation of staining reagents for detection and screening of a large number of different T cell specificities. Paired with the wide range of different MHC class I alleles available at imusyn, we are able to offer T cell staining reagents for virtually any of your need.
Competitive Efficiency
Our Dyemers & myDyemers detect antigen-specific CD8+ T cells reliably and stain with a competitive efficiency when compared to other T cell staining reagents, such as tetramers (Figure 1). Convince yourself and order your T cell staining reagent now.
Figure 1: Staining efficiency of imusyn’s Dyemer compared to a tetramer of a competitor. Staining has been performed on healthy donor PBMCs gated for CD8+ T cells and incubated with PE-labeled Dyemer or tetramer of the same specificity.
Verification of Peptide Binding
Are you unsure, whether your peptide will bind to certain HLA class I alleles or do you want to verify your binding prediction? Try our Class I Peptide Binding Analysis - imuPep. By loading our recombinant HLA class I molecules with your peptides, we can give information on peptide binding by monitoring the thermal stability of the peptide/HLA class I complex.
Upon request, we can provide you with custom-made Dyemers loaded with your confirmed peptides. For requests, please use our contact form.
Order Now
All Dyemers & myDyemers in our Basic Panel are available on demand (see below), including peptide/MHC combinations covering the most prominent and widely used EBV and HCMV specificities.
For orders or quotes, please refer to our order form.
Click here for additional information on peptide origin.
Haven't found your favourite MHC class I alleles in our Basic Panel?
Have a look at our Extended Panel and discover imusyn's diverse range of MHC class I alleles available for myDyemers. For orders or quotes, please refer to our request form.